Western Blot:Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma.
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37- AR TI CL E IN P RE SS 1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000); Peroxidase−AffiniPure Goat Anti−Mouse IgG(H+L) (Jackson Immunoresearch, Cat# 115−035−003, USA, used at 1:10000); Peroxidase IgG Fraction Monoclonal Mouse Anti−Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211−032−171, used at 1:10000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000);..
Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37-1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat anti-mouse IgG(H + L) (Jackson Immunoresearch, Cat# 115-035-003, USA, used at 1:10,000); Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211-032-171, used at 1:10,000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat..
Control:Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma.
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37- AR TI CL E IN P RE SS 1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000); Peroxidase−AffiniPure Goat Anti−Mouse IgG(H+L) (Jackson Immunoresearch, Cat# 115−035−003, USA, used at 1:10000); Peroxidase IgG Fraction Monoclonal Mouse Anti−Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211−032−171, used at 1:10000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000);..
Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37-1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat anti-mouse IgG(H + L) (Jackson Immunoresearch, Cat# 115-035-003, USA, used at 1:10,000); Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211-032-171, used at 1:10,000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat..
Knockdown:Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma.
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37- AR TI CL E IN P RE SS 1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000); Peroxidase−AffiniPure Goat Anti−Mouse IgG(H+L) (Jackson Immunoresearch, Cat# 115−035−003, USA, used at 1:10000); Peroxidase IgG Fraction Monoclonal Mouse Anti−Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211−032−171, used at 1:10000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000);..
Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37-1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat anti-mouse IgG(H + L) (Jackson Immunoresearch, Cat# 115-035-003, USA, used at 1:10,000); Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211-032-171, used at 1:10,000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat..
Transfection:Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma.
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37- AR TI CL E IN P RE SS 1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000); Peroxidase−AffiniPure Goat Anti−Mouse IgG(H+L) (Jackson Immunoresearch, Cat# 115−035−003, USA, used at 1:10000); Peroxidase IgG Fraction Monoclonal Mouse Anti−Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211−032−171, used at 1:10000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000);..
Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37-1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat anti-mouse IgG(H + L) (Jackson Immunoresearch, Cat# 115-035-003, USA, used at 1:10,000); Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211-032-171, used at 1:10,000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat..
Staining:Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma.
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37- AR TI CL E IN P RE SS 1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000); Peroxidase−AffiniPure Goat Anti−Mouse IgG(H+L) (Jackson Immunoresearch, Cat# 115−035−003, USA, used at 1:10000); Peroxidase IgG Fraction Monoclonal Mouse Anti−Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211−032−171, used at 1:10000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000);..
Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37-1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat anti-mouse IgG(H + L) (Jackson Immunoresearch, Cat# 115-035-003, USA, used at 1:10,000); Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211-032-171, used at 1:10,000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat..
Flow Cytometry:Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma.
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37- AR TI CL E IN P RE SS 1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000); Peroxidase−AffiniPure Goat Anti−Mouse IgG(H+L) (Jackson Immunoresearch, Cat# 115−035−003, USA, used at 1:10000); Peroxidase IgG Fraction Monoclonal Mouse Anti−Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211−032−171, used at 1:10000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000);..
Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37-1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat anti-mouse IgG(H + L) (Jackson Immunoresearch, Cat# 115-035-003, USA, used at 1:10,000); Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211-032-171, used at 1:10,000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat..
Immunoprecipitation:Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma.
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37- AR TI CL E IN P RE SS 1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000); Peroxidase−AffiniPure Goat Anti−Mouse IgG(H+L) (Jackson Immunoresearch, Cat# 115−035−003, USA, used at 1:10000); Peroxidase IgG Fraction Monoclonal Mouse Anti−Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211−032−171, used at 1:10000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc−73614, USA, used at 1:20000); SLC7A11 (Proteintech, Cat# 18790−1−AP, China, used at 1:3000 ); GPX4 (Proteintech, Cat# 67763−1−Ig, China, used at 1:2000);..
Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).Enzyme-linked immunosorbent assay (ELISA) kit (MEIMIAN, Cat# MM-92625102, China).. USP7/USP47 inhibitor (KKL Med, Cat# 1247825-37-1, USA), Heclin (KKL Med, Cat# 890605-54-6, USA), C25-140 (KKL Med, Cat# 1358099-18-9, USA), IU1 (KKL Med, Cat# 314245-33-5, USA), b-AP15 (KKL Med, Cat# 1009817-63-3, USA), Wu-5 (TargetMol, Cat# 2630378-05-9, China), Degrasyn (TargetMol, Cat# 856243-80-6, China), BAP1-IN-1 (TargetMol, Cat# 353495-21-3, China), LS-102 (Ambeed, Cat# A1340566, China) and USP8-IN-3 (KKL Med, Cat# 2477651-10-6, USA).. Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat anti-mouse IgG(H + L) (Jackson Immunoresearch, Cat# 115-035-003, USA, used at 1:10,000); Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific (Jackson Immunoresearch, USA, Cat# 211-032-171, used at 1:10,000).Antibodies used in this study are as follows: Vinculin (Santa Cruz, Cat# sc-73614, USA, used at 1:20,000); SLC7A11 (Proteintech, Cat# 18790-1-AP, China, used at 1:3000); GPX4 (Proteintech, Cat# 67763-1-Ig, China, used at 1:2000); Peroxidase-AffiniPure goat..
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